Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
mouse embryonic stem cells
strain
C57BL/6x129sV
passages
8-12
chip antibody
none
cell line
WT26

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated, resuspended in RIPA Lysis Buffer, sheared using Diagenode Bioruptor Plus Sonicator and chromatin was immunoprecipitated with the respective antibodies. Library preparation was carried out following Illumina standard protocols for paired-end sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
91051218
Reads aligned (%)
77.9
Duplicates removed (%)
35.8
Number of peaks
337 (qval < 1E-05)

mm9

Number of total reads
91051218
Reads aligned (%)
77.7
Duplicates removed (%)
36.4
Number of peaks
358 (qval < 1E-05)

Base call quality data from DBCLS SRA