Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
Fetal naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Fetal spleenocytes
tissue
Fetal spleenocytes
age
23.0 weeks
Sex
male
sequencing adapter
ACAGTG
cell type
fetal naive T cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were sorted by FACS, and DNA was extracted from lysed cells following conventional ATACseq and ChIPseq protocols ATACseq libraries were constructed using the standard Illumina Nextera protocol. H3K27ac ChIPseq libraries were constructed using a standard Illumina protocol using Truseq adapters.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
27841942
Reads aligned (%)
98.5
Duplicates removed (%)
7.6
Number of peaks
981 (qval < 1E-05)

hg19

Number of total reads
27841942
Reads aligned (%)
97.4
Duplicates removed (%)
8.7
Number of peaks
986 (qval < 1E-05)

Base call quality data from DBCLS SRA