Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
PBMC
Tissue
blood
Lineage
mesoderm
Description
peripheral blood mononuclear cells

Attributes by original data submitter

Sample

source_name
Human PBMCs
tissue
Human PBMCs
age
50 years
Sex
female
sequencing adapter
CAGATC
cell type
adult CD4+CD25hiCD127lo regulatory T

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were sorted by FACS, and DNA was extracted from lysed cells following conventional ATACseq and ChIPseq protocols ATACseq libraries were constructed using the standard Illumina Nextera protocol. H3K27ac ChIPseq libraries were constructed using a standard Illumina protocol using Truseq adapters.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
43679535
Reads aligned (%)
98.4
Duplicates removed (%)
14.1
Number of peaks
22098 (qval < 1E-05)

hg19

Number of total reads
43679535
Reads aligned (%)
97.9
Duplicates removed (%)
14.7
Number of peaks
22206 (qval < 1E-05)

Base call quality data from DBCLS SRA