Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Gonad
Cell type
TOV-21G
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
TOV21G cell line
cell line
parental TOV21G cell line
chip antibody
anti-Flag (Sigma, catalog# F1804, lot# SLBT7654) antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Flag (Sigma, F1804) antibody was used on TOV21G and resistant endogenously Flag-tagged BRG1 cells. Chromatin were incubated overnight at 4°C and protein A+G Dynabeads were added to the reaction for another 1.5 hour. Magnetic beads were washed and chromatin was eluted and reversed. Chromatin was then treated with proteinase K and purified with Gel extraction kit (Qiagen, cat. no. 28706). ChIP DNA was used for ChIP-seq. For CHIP-seq NEBNext Ultra DNA Library Prep Kit (NEB, E7645) was used to prepare sequencing library

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
32042674
Reads aligned (%)
97.6
Duplicates removed (%)
4.1
Number of peaks
1606 (qval < 1E-05)

hg19

Number of total reads
32042674
Reads aligned (%)
97.0
Duplicates removed (%)
5.4
Number of peaks
1529 (qval < 1E-05)

Base call quality data from DBCLS SRA