Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

U87_shPRMT2_input

cell line

U87

cell type

glioblastoma cells

transfected with

shPRMT2 lentivirus

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Construction of ChIP-seq library was completed according to Illumina's instructions accompanying the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs #E7370)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

9096166

Reads aligned (%)

97.6

Duplicates removed (%)

6.2

Number of peaks

228 (qval < 1E-05)

hg19

Number of total reads

9096166

Reads aligned (%)

96.8

Duplicates removed (%)

7.6

Number of peaks

357 (qval < 1E-05)