GSM2991158: U87 shPRMT2 H3R8me2a; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3R8me2
Cell type
Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma
Attributes by original data submitter
Sample
source_name
U87_shPRMT2_H3R8me2a
cell line
U87
cell type
glioblastoma cells
transfected with
shPRMT2 lentivirus
chip antibody
H3R8me2a (Novus Biologicals, Cat#NB21-1062, Lot A-1)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Construction of ChIP-seq library was completed according to Illumina's instructions accompanying the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs #E7370)