Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3R8me2

Cell type

Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
U87_scramble_H3R8me2a
cell line
U87
cell type
glioblastoma cells
transfected with
scramble control
chip antibody
H3R8me2a (Novus Biologicals, Cat#NB21-1062, Lot A-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Construction of ChIP-seq library was completed according to Illumina's instructions accompanying the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs #E7370)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
17198043
Reads aligned (%)
94.6
Duplicates removed (%)
5.4
Number of peaks
137 (qval < 1E-05)

hg19

Number of total reads
17198043
Reads aligned (%)
94.0
Duplicates removed (%)
6.1
Number of peaks
149 (qval < 1E-05)

Base call quality data from DBCLS SRA