S2 cells were fixed in 1% formaldehyde for 15 min at 18°C. The reaction was stopped by adding glycine to a final concentration of 125 mM. After washing, cells were resuspended in SDS Lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl (pH 8.1), protease inhibitors) and incubated 10 min on ice. Chromatin was prepared by shearing with Bioruptor (Diagenode) at setting “high” for 20 cycles with 30 sec “On” and 30 sec “Off” to yield chromatin with a size ranging from 200-800 bp. The sonicated chromatin was diluted 10 fold in dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl (pH 8.1), 167 mM NaCl, protease inhibitors). Chromatin was immunoprecipitated by incubating with the appropriate antibodies overnight at 4°C. To collect the antibody/chromatin complexes the solution is incubated for 1 h at 4°C with 30 µl Protein G Plus/Protein A Agarose Suspension (Millipore). After washing one time with Low Salt Buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8.1), 150 mM NaCl), one time with High Salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8.1), 500 mM NaCl), one time with LiCl Buffer (0.25 M LiCl, 1% NP40, 1% deoxycholate, 1 mM EDTA, 10 mM Tris-HCl (pH 8.1)) and two times with TE Buffer (10 mM Tris-HCl, 1 mM EDTA (pH 8.0)) the crosslinks were reversed and DNA is recovered by illustra GFX columns (GE Healthcare) Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas instructions. Cluster generation was performed using the Illumina cluster station, sequencing on the Genome Analyzer IIx followed a standard protocol. The fluorescent images were processed to sequences using the Genome Analyzer Pipeline Analysis software 1.8 (Illumina).