Antigen

Antigen Class

TFs and others

Antigen

TP53

Cell type

Cell type Class

Blood

Cell type

T cells

NA

NA

Sample

source_name

PHA-stimulated human T-lymphocytes from healthy volunteer # 104

subject id

104

cell type

PHA-stimulated human T-lymphocytes

chip ab

p53 DO-1 (Santa Cruz, sc-126)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

17-30 million cells were used per each ChIP-seq experiment. Treated cells were crosslinked with 1% formaldehyde, then cells were lysed and nuclei were collected. Chromatin was sheared and immunoprecipitated with p53 DO-1 Ab overnight. Input DNA and p53 ChIP-DNA were reverse crosslinked and purified. Libraries were prepared the using the Illumina ChIP-seq DNA Sample Prep Kit as per manufacturer's instructions .

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

28757194

Reads aligned (%)

96.2

Duplicates removed (%)

9.7

Number of peaks

851 (qval < 1E-05)

hg19

Number of total reads

28757194

Reads aligned (%)

95.2

Duplicates removed (%)

10.7

Number of peaks

1075 (qval < 1E-05)