Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Blood
Cell type
T cells
NA
NA

Attributes by original data submitter

Sample

source_name
PHA-stimulated human T-lymphocytes from healthy volunteer # 45
subject id
45
cell type
PHA-stimulated human T-lymphocytes
chip ab
p53 DO-1 (Santa Cruz, sc-126)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
17-30 million cells were used per each ChIP-seq experiment. Treated cells were crosslinked with 1% formaldehyde, then cells were lysed and nuclei were collected. Chromatin was sheared and immunoprecipitated with p53 DO-1 Ab overnight. Input DNA and p53 ChIP-DNA were reverse crosslinked and purified. Libraries were prepared the using the Illumina ChIP-seq DNA Sample Prep Kit as per manufacturer's instructions .

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
30027804
Reads aligned (%)
89.5
Duplicates removed (%)
6.7
Number of peaks
3001 (qval < 1E-05)

hg38

Number of total reads
30027804
Reads aligned (%)
90.4
Duplicates removed (%)
6.2
Number of peaks
2949 (qval < 1E-05)

Base call quality data from DBCLS SRA