GSM2988929: Lymphocyte-45 DMSO input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
T cells
NA
NA
Attributes by original data submitter
Sample
source_name
PHA-stimulated human T-lymphocytes from healthy volunteer # 45
subject id
45
cell type
PHA-stimulated human T-lymphocytes
chip ab
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
17-30 million cells were used per each ChIP-seq experiment. Treated cells were crosslinked with 1% formaldehyde, then cells were lysed and nuclei were collected. Chromatin was sheared and immunoprecipitated with p53 DO-1 Ab overnight. Input DNA and p53 ChIP-DNA were reverse crosslinked and purified. Libraries were prepared the using the Illumina ChIP-seq DNA Sample Prep Kit as per manufacturer's instructions .