Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Adult
Cell type
Ovary

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
inpu_DNA_ovaries
genotype
w1118
tissue
ovary
chip antibody
input

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Dissected ovaries were fixed in 1% formaldehyde, lysed and sonicated. Part of the lysate was kept for the input DNA, the rest was incubated with pre-immune serum or with the anti-dLsd1 antibody. Antibody complexes were recovered with a mixture of protein A- and G-Sepharose. The DNA was recovered by phenol-chloroform extraction and ethanol precipitation. For the library preparation, ChIP DNA (10 ng) was blunt-ended and phosphorylated, and a single 'A' nucleotide was added to the 3' ends of the fragments in preparation for ligation to an adapter that has a single-base 'T' overhang. The ligation products was purified and size-selected by agencourt AMPure XP beads. Purified DNA was PCR-amplified to enrich for fragments that have adapters on both ends.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
29727205
Reads aligned (%)
86.2
Duplicates removed (%)
10.5
Number of peaks
982 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA