Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2 cells

cell line

HepG2

shRNA

SETD2 shRNA

genotype

SETD2 knockdown

chip antibody

none

replicate

1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysate was sonicated and clarified by using 0.22 µm filter. Histone-DNA complexes were isolated with H3K36me3 antibody. Libraries were constructed by using Auto iDeal ChIP-seq kit for Histones (Diagenode, C01010057) following manufacturer's prorocol.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

49145417

Reads aligned (%)

97.3

Duplicates removed (%)

11.7

Number of peaks

1122 (qval < 1E-05)

hg19

Number of total reads

49145417

Reads aligned (%)

96.6

Duplicates removed (%)

12.9

Number of peaks

1177 (qval < 1E-05)