Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMAD3

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549_Smad3
ttf-1 expression
negative
cell line
A549
histology
lung adenocarcinoma
chip antibody
SMAD3 [Abcam, Catalog#: ab4085, Lot#: GR27326-2]

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP were performed as described (Koinuma et al, Mol Cell Biol 29: 172-186, 2009). SMAD3, SMAD4, or TTF-1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
38151750
Reads aligned (%)
80.4
Duplicates removed (%)
4.6
Number of peaks
5475 (qval < 1E-05)

hg38

Number of total reads
38151750
Reads aligned (%)
82.2
Duplicates removed (%)
3.3
Number of peaks
7299 (qval < 1E-05)

Base call quality data from DBCLS SRA