Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMAD3

Cell type

Cell type Class
Lung
Cell type
NCI-H441
Primary Tissue
Lung
Tissue Diagnosis
Adenocarcinoma Papillary

Attributes by original data submitter

Sample

source_name
H441_Smad3_siTTF-1
ttf-1 expression
depleted by siRNA
cell line
H441
histology
lung adenocarcinoma
chip antibody
SMAD3 [Abcam, Catalog#: ab4085, Lot#: GR27326-2]

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP were performed as described (Koinuma et al, Mol Cell Biol 29: 172-186, 2009). SMAD3, SMAD4, or TTF-1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
32652782
Reads aligned (%)
82.5
Duplicates removed (%)
6.8
Number of peaks
36205 (qval < 1E-05)

hg38

Number of total reads
32652782
Reads aligned (%)
84.0
Duplicates removed (%)
5.7
Number of peaks
36217 (qval < 1E-05)

Base call quality data from DBCLS SRA