Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Prostate
Cell type
LREX
NA
NA

Attributes by original data submitter

Sample

source_name
IP:GR with Dex treatment
cell line
LREX'
cell type
Prostate Cancer
phenotype
Enzalutamide Resistant

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixation and processing for was carried out as described by others (Goldberg et al., 2010). Immunoprecipitation was carried out with Anti-Androgen Receptor Antibody, PG-21 (Millipore) or Glucocorticoid Receptor Antibody #7437 (Cell Signaling). Immunoprecipitated DNA was quantified by picogreen and size was evaluated on a HighSense BioAnalyzer chip. Fragments between 100 and 600 bp were collected using an automated system (Pippin Prep, Sage Science) then end repaired, ligated and amplified for 15 cycles using reagents included in the Truseq DNA Sample Preparation kit from Illumina. Experimental conditions followed strictly the instructions of the manufacturer, with the exception of the adaptors being diluted 1/10 for the input DNA and 1/50 for all other samples.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
18125354
Reads aligned (%)
106.1
Duplicates removed (%)
24.7
Number of peaks
5002 (qval < 1E-05)

hg38

Number of total reads
18125354
Reads aligned (%)
108.1
Duplicates removed (%)
24.3
Number of peaks
5069 (qval < 1E-05)

Base call quality data from DBCLS SRA