GSM2978424: ChIPSeq CBX7 UNC4976 4h rep; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Lymphocytes
cell type
CD4+ T cells
treatment condition
UNC4976 (4h)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
We fixed 10-20 million CD4 T lymphocytes, after 4h treatment with 20 mM UNC4976 (0.2% DMSO) or 0.2% DMSO, with 1% formaldehyde for 12 minutes. After fixation, formaldehyde was quenched by adding glycine to a final concentration of 100 mM. The Fixed cells were washed in ice cold PBS, pelleted at 1200g and after decanting remaining PBS, the pellets were flash frozen with liquid nitrogen. After harvesting fixed nuclei and chromatin was sheared and immunoprecipitated as previously described (Hodges and Stanton et al Nature Struct Mol Biol 2017). For these ChIP studies, rabbit anti-CBX7 antibody (Abcam cat# 21873, Lot number: GR255463-2) was used. After reverse crosslinking, ChIP DNA was phenol-chloroform extracted. Library preparation was performed as previously described (Love et al. Genome Biol. 2014)