Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Epidermis

Cell type

BJ

Primary Tissue

Skin

Tissue Diagnosis

Normal

Sample

source_name

BJ Fibroblasts

cell line

BJ Fibroblast

chip antibody

H3K4me1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were generated as previously described (Mikkelsen et al., 2010, Gifford et al., 2013) and Libraries were sequenced on a HiSeq 2500 at 11pmol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

17408835

Reads aligned (%)

84.2

Duplicates removed (%)

2.5

Number of peaks

611 (qval < 1E-05)

hg19

Number of total reads

17408835

Reads aligned (%)

83.4

Duplicates removed (%)

4.0

Number of peaks

780 (qval < 1E-05)