Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549

cell line

A549

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were generated as previously described (Mikkelsen et al., 2010, Gifford et al., 2013) and Libraries were sequenced on a HiSeq 2500 at 11pmol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

125433074

Reads aligned (%)

98.5

Duplicates removed (%)

3.5

Number of peaks

1670 (qval < 1E-05)

hg19

Number of total reads

125433074

Reads aligned (%)

97.7

Duplicates removed (%)

3.9

Number of peaks

1382 (qval < 1E-05)