Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PRMT1

Cell type

Cell type Class
Epidermis
Cell type
Keratinocytes
MeSH Description
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Attributes by original data submitter

Sample

source_name
human keratinoctyes expressing HA-PRMT1
cell type
primary human neonatal keratinocytes
antibody
HA (cell signaling C29F4)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, HA-PRMT1 expressing primary keratinocytes were crosslinked with 1% formaldehyde and DSG, sonicated, and immunoprecipiated with antibodies recognizing the HA tag. For ChIP-seq, NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina was used for library construction.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
69755508
Reads aligned (%)
70.0
Duplicates removed (%)
28.0
Number of peaks
1889 (qval < 1E-05)

hg38

Number of total reads
69755508
Reads aligned (%)
71.7
Duplicates removed (%)
26.8
Number of peaks
2089 (qval < 1E-05)

Base call quality data from DBCLS SRA