Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
INTS3

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa
cell line
HeLa
antibody
16620-1-AP: INTS3 antibody, Rabbit Polyclonal. Proteintech
knockdown
siSpt6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nascent RNAs were purified from Pol II IP products of solublilized chromatin. DNA were purified from chromatin IP products. Libraries were prepared according to the protocols of NEBNext small RNA library preparation kit (NEB) for mNET-seq; NEBNext Ultra II Directional RNA Library preparation kit (NEB) for Chromatin, Nucleoplasmic, Cytoplasmic and Nuclear poly(A)+ RNA-seq and RDIP-seq; QuantSeq 3' mRNA-Seq library preparation kit REV for Illumina (Lexogen) for 3'RNA-seq; NEBNext Ultra II DNA Library preparation kit (NEB) for mNucD-seq and ChIP-seq

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
54017633
Reads aligned (%)
177.3
Duplicates removed (%)
8.5
Number of peaks
2939 (qval < 1E-05)

hg38

Number of total reads
54017633
Reads aligned (%)
179.0
Duplicates removed (%)
8.1
Number of peaks
3525 (qval < 1E-05)

Base call quality data from DBCLS SRA