Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RELA

Cell type

Cell type Class
Kidney
Cell type
Renal cell carcinoma
NA
NA

Attributes by original data submitter

Sample

source_name
786-0_NFkB-p65_ChIP
cell line
786-0
cell type
clear cell renal cell carcinoma (ccRCC) cell line
chip antibody
p65 (Santa Cruz Biotechnology, sc-372-x)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with ZHX2 (Genetex, 112232) or p65 (Santa Cruz Biotechnology, sc-372-x) antibodies as previously described (37, 38). TruSeq libraries were constructed as per manufacturer's recommendations.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
17250945
Reads aligned (%)
86.4
Duplicates removed (%)
6.7
Number of peaks
8603 (qval < 1E-05)

hg38

Number of total reads
17250945
Reads aligned (%)
87.2
Duplicates removed (%)
6.3
Number of peaks
8660 (qval < 1E-05)

Base call quality data from DBCLS SRA