Antigen

Antigen Class

Histone

Antigen

H3K9ac

Cell type

Cell type Class

Blood

Cell type

ALL xenograft

NA

NA

Sample

source_name

ALL xenograft cells

tissue

ALL xenograft cells

treatment

Dex

chip antibody

H3K9Ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell suspensions of spleens were prepared and mononuclear cells enriched to >97% human by density gradient centrifugation. The spleen-harvested cells were used for ChIP-seq assay. Lysates were clarified from sonicated nuclei and crosslinked protein-DNA complexes were isolated with antibody. Library preparation was performed by Novogene (Hong Kong) using a variation of the Illumina's standard protocol. Briefly, the pipeline consists of 3 steps; 1. DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptors; 2. PCR amplification and size selection (usually 100-300bp, including adaptor sequence); 3. Qualified library for cluster preparation and sequencing.

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

21986706

Reads aligned (%)

90.9

Duplicates removed (%)

7.5

Number of peaks

13431 (qval < 1E-05)

hg19

Number of total reads

21986706

Reads aligned (%)

90.5

Duplicates removed (%)

8.0

Number of peaks

13489 (qval < 1E-05)