GSM2973237: SCC25 ETS1 C20 Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ETS1
Cell type
Cell type Class
Others
Cell type
SCC-25
Primary Tissue
Tongue
Tissue Diagnosis
Carcinoma Squamous Cell
Attributes by original data submitter
Sample
source_name
SCC25
cell line
SCC25
antibody
C20 (Santa Cruz) antibodies for ETS1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted from Mesenchymal HNSCC Cell Line (SCC25) using a low SDS shearing kit (Diagenode: C01020010). Lysates were sonicated with a Bioruptor for 30-40 min. IPs were carried out using the Active-Motif High Sensitivity ChIP Kit using the C20 (Santa Cruz) and 501A (Bethyl Labs) antibodies for ETS1. Libraries were prepared using ThruPLEX DNA-seq kits from Rubicon Genomics. The samples were then 50 bp single-end sequenced on an Illumina HiSeq 2500.