Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Uterus
Cell type
Ishikawa
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Ishikawa
cell line
Ishikawa
treatment
100 nM Dexamethasone for 1 hour
target protein
GR
antibody
GR E-20 (Santa Cruz sc-1003)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature and then treated with 125 mM Glycine for 5 minutes to stop crosslinking. Crosslinked cells were then washed with cold PBS and scraped to harvest. ChIP and library construction was performed as described in Reddy et al, Genome Research, 2009

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
20739270
Reads aligned (%)
42.9
Duplicates removed (%)
12.0
Number of peaks
5243 (qval < 1E-05)

hg38

Number of total reads
20739270
Reads aligned (%)
43.7
Duplicates removed (%)
11.1
Number of peaks
5207 (qval < 1E-05)

Base call quality data from DBCLS SRA