GSM1242276: LoVoSTTnocodazole0 RAD21 Rabbit; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RAD21
Cell type
Cell type Class
Digestive tract
Cell type
LoVo
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
LoVo
cell line
LoVo
cell type
colon adenocarcinoma
hgn
RAD21
antibody
Santa Cruz Biotechnology, Inc. : sc-98784
control sample
LoVoSTTnocodazole0_IgG_Rabbit
blocking protocol
Thymidine block + nocodazole
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked by 1% formaldehyde, and DNA was sonicated to 200- 400 bp fragments. Antibodies (5 µg) were added and collected using protein G Sepharose (GE). Cross-links were reversed and proteins digested by incubation with proteinase K at 65 °C overnight. DNA was then purified using Qiagen PCR purification kit.