Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Digestive tract

Cell type

GP5d

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

GP5d

cell line

GP5d

cell type

colon adenocarcinoma

hgn

H3K4me1

antibody

Abcam : ab1791

control sample

GP5dsiRAD21_IgG_Rabbit

sirna

RAD23

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked by 1% formaldehyde, and DNA was sonicated to 200- 400 bp fragments. Antibodies (5 µg) were added and collected using protein G Sepharose (GE). Cross-links were reversed and proteins digested by incubation with proteinase K at 65 °C overnight. DNA was then purified using Qiagen PCR purification kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

13495122

Reads aligned (%)

93.8

Duplicates removed (%)

3.3

Number of peaks

10999 (qval < 1E-05)

hg19

Number of total reads

13495122

Reads aligned (%)

93.5

Duplicates removed (%)

3.4

Number of peaks

11011 (qval < 1E-05)