Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

Chromatin IP against H3K4me3

chip antibody

H3K4me3 (Millipore, #07-473)

cell line

MCF10A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were sonicated to retrieve lysates and protein-DNA complexes were isolated with antibody. To make libraries, TruSeq protocol was followed

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

32892546

Reads aligned (%)

93.4

Duplicates removed (%)

21.8

Number of peaks

36087 (qval < 1E-05)

hg19

Number of total reads

32892546

Reads aligned (%)

93.1

Duplicates removed (%)

22.2

Number of peaks

36035 (qval < 1E-05)