Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
H3K27Ac_HUVEC_siERG+siFLI1
cell type
HUVEC
stimulation
siERG+siFLI1, day7
chip antibody
H3K27Ac (MBL, MABI0309)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysate was sonicated and subsequently incubated with the antibody to isolate DNA. Libraries were prepared using KAPA Hyper Prep Kit (for illumina) (KAPA)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
31848741
Reads aligned (%)
83.9
Duplicates removed (%)
9.0
Number of peaks
58598 (qval < 1E-05)

hg19

Number of total reads
31848741
Reads aligned (%)
83.8
Duplicates removed (%)
9.1
Number of peaks
58395 (qval < 1E-05)

Base call quality data from DBCLS SRA