Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

H3K27Ac_HUVEC_siControl

cell type

HUVEC

stimulation

siControl, day7

chip antibody

H3K27Ac (MBL, MABI0309)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysate was sonicated and subsequently incubated with the antibody to isolate DNA. Libraries were prepared using KAPA Hyper Prep Kit (for illumina) (KAPA)

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

31635611

Reads aligned (%)

96.3

Duplicates removed (%)

16.7

Number of peaks

31012 (qval < 1E-05)

hg19

Number of total reads

31635611

Reads aligned (%)

95.8

Duplicates removed (%)

17.2

Number of peaks

30934 (qval < 1E-05)