Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
H3K4me3_HUVEC_siERG+siFLI1
cell type
HUVEC
stimulation
siERG+siFLI1, day7
chip antibody
H3K4me3 (MBL, MABI0304)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysate was sonicated and subsequently incubated with the antibody to isolate DNA. Libraries were prepared using KAPA Hyper Prep Kit (for illumina) (KAPA)

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
36291652
Reads aligned (%)
95.8
Duplicates removed (%)
22.6
Number of peaks
23347 (qval < 1E-05)

hg19

Number of total reads
36291652
Reads aligned (%)
95.3
Duplicates removed (%)
23.2
Number of peaks
23499 (qval < 1E-05)

Base call quality data from DBCLS SRA