Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Multiple Myeloma

MeSH Description

A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Sample

source_name

multiple myeloma

tissue

peripheral blood

cell type

B lymphocyte

antibody

H3K27me3 (Millipore, 07-449)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cell and histone-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina MiSeq

hg38

Number of total reads

12898983

Reads aligned (%)

99.4

Duplicates removed (%)

0.8

Number of peaks

184 (qval < 1E-05)

hg19

Number of total reads

12898983

Reads aligned (%)

98.6

Duplicates removed (%)

3.1

Number of peaks

198 (qval < 1E-05)