Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
C2C12 myoblasts
strain
C3H
chip antibody
none
cell line
C2C12

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in formaldehyde and sonicated with a Covaris S220 Ultra-Sonicator system. DNA was precipitated with the appropriate antibody and sent for sequencing to the McGill University Genome Quebec Innovation Centre using Illumina HiSeq 2000 platform. Genomic DNA libraries were prepared for sequencing by McGill University Genome Quebec Innovation Centre using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50228024
Reads aligned (%)
96.8
Duplicates removed (%)
13.7
Number of peaks
496 (qval < 1E-05)

mm9

Number of total reads
50228024
Reads aligned (%)
96.6
Duplicates removed (%)
13.7
Number of peaks
597 (qval < 1E-05)

Base call quality data from DBCLS SRA