Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

Human Endothlial cell line

cell type

Primary Human Umbilical Vein Endothelial Cells

passage

P3-6

antibody

H3K27ac, Abcam

agent

VEGF

time point

12hour

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChiP-seq: Chromatin DNA was extracted from sonicated nuclei and further selectively accumulated by ChiP-specific antibody. RNA-seq: total RNA were purified by Qiagen RNeasy column. Illumina multiplex library protocol for ChiP-seq and mRNA seq of VEGF stimualtion is as described (Bing Zhang, et al. , 2013,Genome Res. 2013 Jun;23(6):917-27); Spike-in mRNA-seq protocol followed ScriptSeq v2 RNA-seq kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

45632862

Reads aligned (%)

99.5

Duplicates removed (%)

11.9

Number of peaks

64129 (qval < 1E-05)

hg19

Number of total reads

45632862

Reads aligned (%)

99.0

Duplicates removed (%)

12.5

Number of peaks

64696 (qval < 1E-05)