Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Human Endothlial cell line
cell type
Primary Human Umbilical Vein Endothelial Cells
passage
P3-6
antibody
H3K4me2, Abcam
agent
VEGF
time point
0hour

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChiP-seq: Chromatin DNA was extracted from sonicated nuclei and further selectively accumulated by ChiP-specific antibody. RNA-seq: total RNA were purified by Qiagen RNeasy column. Illumina multiplex library protocol for ChiP-seq and mRNA seq of VEGF stimualtion is as described (Bing Zhang, et al. , 2013,Genome Res. 2013 Jun;23(6):917-27); Spike-in mRNA-seq protocol followed ScriptSeq v2 RNA-seq kit (Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
19510107
Reads aligned (%)
98.8
Duplicates removed (%)
7.2
Number of peaks
56425 (qval < 1E-05)

hg19

Number of total reads
19510107
Reads aligned (%)
98.5
Duplicates removed (%)
7.6
Number of peaks
56268 (qval < 1E-05)

Base call quality data from DBCLS SRA