Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

KG-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

KG1 cell line

cell line

KG1

tissue

bone marrow

culture properties

suspension

genotype

shPLZF

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA or proteins-DNA complexes were isolated with antibody. DNA was purified using the i-Pure kit (Diagenode) Libraries were prepared according to Illumina's instructions accompanying the Illumina's TruSeq ChIP sample Prep Kit Protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

63394818

Reads aligned (%)

94.9

Duplicates removed (%)

4.3

Number of peaks

1573 (qval < 1E-05)

hg19

Number of total reads

63394818

Reads aligned (%)

94.3

Duplicates removed (%)

5.4

Number of peaks

1560 (qval < 1E-05)