Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
KG-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
KG1 cell line
cell line
KG1
tissue
bone marrow
culture properties
suspension
genotype
wild type
antibody
H3K27me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA or proteins-DNA complexes were isolated with antibody. DNA was purified using the i-Pure kit (Diagenode) Libraries were prepared according to Illumina's instructions accompanying the Illumina's TruSeq ChIP sample Prep Kit Protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
76994877
Reads aligned (%)
97.2
Duplicates removed (%)
9.0
Number of peaks
5043 (qval < 1E-05)

hg19

Number of total reads
76994877
Reads aligned (%)
96.5
Duplicates removed (%)
10.5
Number of peaks
4796 (qval < 1E-05)

Base call quality data from DBCLS SRA