Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Liver

Cell type

HuH-7

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HuH-7 cells

cell type

Liver cancer cells

chip antibody

H3K4me3 (CST, 9751)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

86479386

Reads aligned (%)

42.8

Duplicates removed (%)

21.6

Number of peaks

39211 (qval < 1E-05)

hg19

Number of total reads

86479386

Reads aligned (%)

3.0

Duplicates removed (%)

29.9

Number of peaks

54 (qval < 1E-05)