GSM2946596: Control Input DNA Dox Minus, 12hr; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
G3-hESCs
treatment
Doxycycline Minus
cell line
H9 hESCs with Dox inducible Glis3 expression
time
12 hr
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with A/G-conjugated magnetic beads with a HA-antibody. ChIPed DNA was amplified using a TruSeq ChIP Library prep kit.