Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEF2C

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
actue myeloid luekemia
cell line
MOLM-13 cell
treatment
none
chip antibody
MEF2C (Cell Signaling Technologies, 5030S)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
30254290
Reads aligned (%)
75.1
Duplicates removed (%)
18.1
Number of peaks
58008 (qval < 1E-05)

hg38

Number of total reads
30254290
Reads aligned (%)
76.2
Duplicates removed (%)
17.3
Number of peaks
58245 (qval < 1E-05)

Base call quality data from DBCLS SRA