GSM2944365: H3K27ac HEL sgSIK3e4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
HEL
Primary Tissue
Blood
Tissue Diagnosis
Erythroleukemia
Attributes by original data submitter
Sample
source_name
actue myeloid luekemia
cell line
HEL cell
treatment
day 5 after sgSIK3e4 guideRNA infection
chip antibody
H3K27ac (abcam ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.