Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cardiovascular
Cell type
Heart Ventricles
MeSH Description
The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.

Attributes by original data submitter

Sample

source_name
Adult_SrfChIP_input
genotype/variation
Srf-fbio/+;Rosa-birA/+
age
postnatal day 42
tissue
heart ventricle
chip method
strepavidin pull-down of biotinylated SRF

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Heart ventricles were homogenized and fixed by 1% formaldehyde, chromatin isolated and sheared by sonication SRF bound DNA was pulled down onto Streptavidin beads. Purified DNA released from beads was converted into sequencing libraries by Kapa Hyper Library Kit

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
22259219
Reads aligned (%)
97.4
Duplicates removed (%)
12.2
Number of peaks
249 (qval < 1E-05)

mm9

Number of total reads
22259219
Reads aligned (%)
97.2
Duplicates removed (%)
12.2
Number of peaks
220 (qval < 1E-05)

Base call quality data from DBCLS SRA