SRX3591448
GSM2942533: H3K27Ac ChIPseq 3c; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
B-CLL cells
NA
NA

Attributes by original data submitter

Sample

source_name
B-Chronic Lymphocytic Leukemia (CLL) cels
cell type
Primary B-CLL cells
protocol
4 hours stimulation with 3.2uM of CpG oligonucleotide + 1 uM of BET inhibitor (PLX51107)
chip-antibody
H3K27ac (Active Motif #39133)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
extract_protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
30967275
Reads aligned (%)
97.6
Duplicates removed (%)
34.9
Number of peaks
22000 (qval < 1E-05)

hg19

Number of total reads
30967275
Reads aligned (%)
97.3
Duplicates removed (%)
35.2
Number of peaks
21910 (qval < 1E-05)

Base call quality data from DBCLS SRA