Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
B-CLL cells
NA
NA

Attributes by original data submitter

Sample

source_name
B-Chronic Lymphocytic Leukemia (CLL) cels
cell type
Primary B-CLL cells
protocol
4 hours stimulation with 3.2uM of CpG oligonucleotide
chip-antibody
H3K27ac (Active Motif #39133)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
extract_protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
32258349
Reads aligned (%)
91.3
Duplicates removed (%)
74.2
Number of peaks
16211 (qval < 1E-05)

hg19

Number of total reads
32258349
Reads aligned (%)
91.1
Duplicates removed (%)
74.5
Number of peaks
16120 (qval < 1E-05)

Base call quality data from DBCLS SRA