Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
B-CLL cells
NA
NA

Attributes by original data submitter

Sample

source_name
B-Chronic Lymphocytic Leukemia (CLL) cels
cell type
Primary B-CLL cells
protocol
4 hours stimulation with 3.2uM of CpG oligonucleotide + 1 uM of BET inhibitor (PLX51107)
chip-antibody
BRD4 (Active Motif #A301-985A1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
extract_protocol Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
33301669
Reads aligned (%)
65.4
Duplicates removed (%)
32.5
Number of peaks
3176 (qval < 1E-05)

hg38

Number of total reads
33301669
Reads aligned (%)
67.4
Duplicates removed (%)
30.9
Number of peaks
3244 (qval < 1E-05)

Base call quality data from DBCLS SRA