Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
Adult Human Peripheral Blood
tissue
Adult Human Peripheral Blood
chip-antibody
H3K4me1
cell type
Macrophages
donor
Buffycoat 11

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIPseq experiments were followed the Blueprint protocol, http://www.blueprint-epigenome.eu/. Libraries were prepared using the KAPA hyperprep kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
61699534
Reads aligned (%)
93.5
Duplicates removed (%)
7.6
Number of peaks
2702 (qval < 1E-05)

hg19

Number of total reads
61699534
Reads aligned (%)
93.2
Duplicates removed (%)
8.3
Number of peaks
2432 (qval < 1E-05)

Base call quality data from DBCLS SRA