Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

Adult Human Peripheral Blood

tissue

Adult Human Peripheral Blood

chip-antibody

H3K4me3

cell type

Monocytes

donor

Buffycoat 19

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIPseq experiments were followed the Blueprint protocol, http://www.blueprint-epigenome.eu/. Libraries were prepared using the KAPA hyperprep kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

35782115

Reads aligned (%)

98.6

Duplicates removed (%)

11.3

Number of peaks

29089 (qval < 1E-05)

hg19

Number of total reads

35782115

Reads aligned (%)

98.2

Duplicates removed (%)

11.4

Number of peaks

29543 (qval < 1E-05)