Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
Lymphoblastoid cell line
Tissue
blood
Lineage
mesoderm
Description
parental cell type to lymphoblastoid cell lines

Attributes by original data submitter

Sample

source_name
LCLs cells
cell type
LCLs cells
antibody
H3K27me3
agent
4-hydroxytamoxifen

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
EBNA3CHT LCLs were maintained in RPMI 1640 supplemented with 10% FBS, L-glutamine, streptomycin, and penicillin and with or without 400 nM 4HT for 3 weeks. LCLs were cross-linked with 1% formaldehyde and immuneprecipitated with H3K27me3 antibody. After extensive washing, Protein-DNA complexes were eluted and cross-linking was reversed. The purified DNA was sequenced using a HiSeq 2500. Libraries were prepared according to Illumina's standard instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
100440500
Reads aligned (%)
96.2
Duplicates removed (%)
14.5
Number of peaks
4112 (qval < 1E-05)

hg19

Number of total reads
100440500
Reads aligned (%)
95.7
Duplicates removed (%)
15.4
Number of peaks
3877 (qval < 1E-05)

Base call quality data from DBCLS SRA