Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
glp-1; set-26_whole_worm
strain background
N2
genotype/variation
glp-1; set-26
developmental stage
L4
chip antibody
H3K4me3 (Millipore, Billerica, MA 17-614, lot:2756917)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-seq: RNA was extracted using Trizol reagent. ChIP-seq: Crosslinked protein-DNA complex were isolated with antibody. RNA libraries were prepared for sequencing using Ovation Human FFPE RNA-Seq Library Systems (NuGEN) kit ChIP-seq libraries were prepared for sequencing using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB)

Sequencing Platform

instrument_model
NextSeq 500

ce11

Number of total reads
32930092
Reads aligned (%)
97.8
Duplicates removed (%)
19.3
Number of peaks
5836 (qval < 1E-05)

ce10

Number of total reads
32930092
Reads aligned (%)
97.8
Duplicates removed (%)
19.3
Number of peaks
5824 (qval < 1E-05)

Base call quality data from DBCLS SRA