Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7L Tamoxifen Resistant
cell types
immortalized breast epithelial cell line
chip antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA extracted using Quick-RNA MiniPrep Kit from ZYMO Research TCC was performed as described (Kalhor et al. 2012). ChIP-seq and RNA-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
65288224
Reads aligned (%)
98.4
Duplicates removed (%)
9.8
Number of peaks
25393 (qval < 1E-05)

hg19

Number of total reads
65288224
Reads aligned (%)
97.6
Duplicates removed (%)
11.4
Number of peaks
25161 (qval < 1E-05)

Base call quality data from DBCLS SRA